Ten siderophore-related compounds were isolated by MS/MS-based molecular networking from a mesophotic sponge-associated Streptomyces diastaticus NBU2966. Among them, there were three kinds of siderophores including three undescribed phenol/thiazoline-type compounds, thiazostatin C, methyl thiazostatin B, and pulicatin J, one undescribed phenol/oxazoline-type compound, spoxazomicin E, and one undescribed phenol/oxazoline/thiazoline-type compound, streptochelin A, as well as five known compounds. Pulicatin J and spoxazomicin E were both isolated as a pair of inseparable epimers. The planar structures of all these compounds were determined based on the detailed NMR and HRESIMS spectroscopic analysis, and the absolute configurations of them were assigned by ECD and NMR quantum chemical calculations. Additionally, in vitro antibacterial activities, inhibition of MRSA biofilm formation, and neuroprotective activity for these compounds were tested.

Depth range
76- 76 m

Mesophotic “mentions”
11 x (total of 8528 words)

Classification
* Presents original data
* Focused on 'mesophotic' depth range
* Focused on 'mesophotic coral ecosystem'

Fields
Biomedicine

Focusgroups
Bacteria and Archaea

Locations
Philippines

Platforms
SCUBA (open-circuit or unspecified)