Two new polyketide natural products, globosuxanthone F (1), and 2'-hydroxy bisdechlorogeodin (2), were isolated from the fungus Pleosporales sp. NBUF144, which was derived from a 62 m deep Chalinidae family sponge together with four known metabolites, 3,4-dihydroglobosuxanthone A (3), 8-hydroxy-3-methylxanthone-1-carboxylate (4), crosphaeropsone C (5), and 4-megastigmen-3,9-dione (6). The structures of these compounds were elucidated on the basis of extensive spectroscopic analysis, including 1D and 2D NMR and high-resolution electrospray ionization mass spectra (HRESIMS) data. The absolute configuration of 1 was further established by single-crystal X-ray diffraction studies. Compounds 1-5 were evaluated for cytotoxicity towards CCRF-CEM human acute lymphatic leukemia cells, and it was found that 1 had an IC50 value of 0.46 µM.

Depth range
62- 62 m

Mesophotic “mentions”
8 x (total of 3673 words)

Classification
* Presents original data
* Focused on 'mesophotic' depth range
* Focused on 'mesophotic coral ecosystem'

Fields
Biomedicine
Molecular ecology

Focusgroups
Porifera (Sponges)
Fungi

Locations
Philippines

Platforms
SCUBA (open-circuit or unspecified)