Publications

Morrow et al. 2016


scientific article | Environ Microbiol | open access Open access small aa108fa7f478951c693af64a05bc4b46e6711dbb69a20809512a129d4d6b870f

Environmental drivers of microbial community shifts in the giant barrel sponge, Xestospongia muta, over a shallow to mesophotic depth gradient

Morrow KM, Fiore Cara, Lesser M

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Abstract

The giant barrel sponge, Xestospongia muta, is a high microbial abundance sponge found on Caribbean coral reefs along shallow to mesophotic depth gradients where multiple abiotic factors change with depth. Sponges were collected along a depth gradient at Little Cayman (LC) and Lee Stocking Island (LSI) and the microbiome of these samples was analyzed using 16S rRNA amplicon sequencing. Statistically significant shifts in community structure and dissimilarity (∼40%) were detected from 10 to 90 m in LC sponges, but a similar shift was not identified in sponges from 10-60 m at LSI (only 17% dissimilar). Additionally, inorganic nutrient levels steadily increased with depth at LSI but not at LC. Based on bulk stable isotopic variability, sponges collected from LC were generally more enriched in 15N and less enriched in 13C as depth increased, suggesting a transition from dependency on photoautotrophy to heterotrophy as depth increased. Patterns of stable isotopic enrichment were largely invariant at LSI, which is also reflected in the more stable microbial community along the depth gradient. It appears that environmental factors that change with depth may contribute to differences in X. muta microbial assemblages, demonstrating the importance of contemporaneous environmental sampling in studies of the microbiome of sponges.

Behind the science
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Research sites

Morrow et al 2016 environmental microbiology
Keywords
Meta-data (pending validation)
Depth range
10- 90 m

Mesophotic “mentions”
23 x (total of 5281 words)

Fields
Community structure Molecular ecology

Research focus
Porifera (Sponges) Bacteria and Archaea

Locations
Cayman Islands Bahamas

Research platforms
Diving - Technical Open-Circuit
Author profiles
Michael P. Lesser ( 14 pubs)
Kathleen Morrow ( 2 pubs)